anti human dsg2 polyclonal antibody af947  (R&D Systems)

Journal: Anatolian Journal of Cardiology

Article Title: Desmosomal Junctions and Connexin-43 Remodeling in High-Pacing-Induced Heart Failure Dogs

doi: 10.14744/AnatolJCardiol.2023.2823

Figure Lengend Snippet: The Primers Used in this Study

Article Snippet: Membranes were incubated with primary antibodies [anti-Cx43 (ab11370, 1:1000, Abcam, USA), anti-DSG2 (bs-10152R, 1:1000, Bioss), GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) (MAB374, 1:2000, Chemicon, USA)] overnight at 4°C followed by secondary antibodies (1:5000, Zhongshanjinqiao, China) at room temperature for 1 hour.

Techniques:

Journal: Anatolian Journal of Cardiology

Article Title: Desmosomal Junctions and Connexin-43 Remodeling in High-Pacing-Induced Heart Failure Dogs

doi: 10.14744/AnatolJCardiol.2023.2823

Figure Lengend Snippet: Immunofluorescent staining for the DP, DSG2, and Cx43. (A) Representative immunofluorescent images of DP, DSG2, and Cx43 in the myocardial. (B) Cx43 and DSG2 distribution. (C) Comparison of Pearson coefficient values for colocalization of Cx43 with various molecules (DSG2, DP) at the LM in the hearts of HF group. Cx43, Connexin-43; DP, Desmoplakin; DSG2, desmoglein-2; HF, heart failure; LM, lateral membrane.

Article Snippet: Membranes were incubated with primary antibodies [anti-Cx43 (ab11370, 1:1000, Abcam, USA), anti-DSG2 (bs-10152R, 1:1000, Bioss), GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) (MAB374, 1:2000, Chemicon, USA)] overnight at 4°C followed by secondary antibodies (1:5000, Zhongshanjinqiao, China) at room temperature for 1 hour.

Techniques: Staining, Comparison, Membrane

Journal: Anatolian Journal of Cardiology

Article Title: Desmosomal Junctions and Connexin-43 Remodeling in High-Pacing-Induced Heart Failure Dogs

doi: 10.14744/AnatolJCardiol.2023.2823

Figure Lengend Snippet: Relative protein expression of DSG2, Cx43. Cx43, DSG2 protein expression by western blot. Cx43, Connexin-43; DSG2, desmoglein-2. ** P < .01 vs. control, *** P < .001 vs. control, n = 6 for each group.

Article Snippet: Membranes were incubated with primary antibodies [anti-Cx43 (ab11370, 1:1000, Abcam, USA), anti-DSG2 (bs-10152R, 1:1000, Bioss), GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) (MAB374, 1:2000, Chemicon, USA)] overnight at 4°C followed by secondary antibodies (1:5000, Zhongshanjinqiao, China) at room temperature for 1 hour.

Techniques: Expressing, Western Blot, Control

Journal: Anatolian Journal of Cardiology

Article Title: Desmosomal Junctions and Connexin-43 Remodeling in High-Pacing-Induced Heart Failure Dogs

doi: 10.14744/AnatolJCardiol.2023.2823

Figure Lengend Snippet: Relative mRNA expression of DSG2, Cx43 by qRT-PCR. * P < . 05 vs. control, ** P < .01 vs. control, * ** P < .001 vs. control, n = 6 for each group.

Article Snippet: Membranes were incubated with primary antibodies [anti-Cx43 (ab11370, 1:1000, Abcam, USA), anti-DSG2 (bs-10152R, 1:1000, Bioss), GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) (MAB374, 1:2000, Chemicon, USA)] overnight at 4°C followed by secondary antibodies (1:5000, Zhongshanjinqiao, China) at room temperature for 1 hour.

Techniques: Expressing, Quantitative RT-PCR, Control

Journal: Blood Advances

Article Title: In vivo HSC transduction in rhesus macaques with an HDAd5/3 + vector targeting desmoglein 2 and transiently overexpressing cxcr4

doi: 10.1182/bloodadvances.2022007975

Figure Lengend Snippet: Analysis of DSG2 expression on HSCs. (A) Flow cytometry for CD46 and DSG2 on human CD34 + cells from 2 different healthy G-CSF–mobilized donors. (B) Schematic of HDAd vector capsids and genomes. The chimeric capsids contain either affinity-enhanced Ad35 or Ad3 fiber knob domains. Both vector genomes contain an EF1α-mgmt p140k /GFP expression cassette used before. Both proteins are linked via a self-cleaving picornavirus 2A peptide. One set of vectors has an additional CMV promoter–human cxcr4 expression cassette. The 4 vectors used in this study are HDAd5/35++GFP, HDAd5/3+GFP, HDAd5/35++GFP/cxcr4, and HDAd5/3+GFP/cxcr4. (C) In vitro CD34 + cell transduction studies. Cells were infected with HDAd5/35++-GFP and HDAd5/3+-GFP virus at a multiplicity of infection of 2000 vp/cell with and without pre-incubation with recombinant soluble CD46 or DSG2 (10 μg/mL) for 1 hour. GFP expression was analyzed 24 hours' postinfection. (D) CD46 and DSG2 flow cytometry on human and rhesus peripheral RBCs stained with antibodies that recognize the receptors in both species (anti-human CD46 mAb clone M177 from Santa Cruz Biotechnology [Dallas, TX] and anti-human DSG2 polyclonal antibody AF947 from R&D Systems [Minneapolis, MN]). (E) Transduction of 293 cells with HDAd5/35++GFP and HDAd5/3+GFP in the absence and presence of human (huRBCs) and rhesus (rhRBCs) RBCs (details are provided in the Materials and methods). Shown is the percentage of GFP-positive cells measured 2 days after transduction.

Article Snippet: GFP expression was analyzed 24 hours' postinfection. (D) CD46 and DSG2 flow cytometry on human and rhesus peripheral RBCs stained with antibodies that recognize the receptors in both species (anti-human CD46 mAb clone M177 from Santa Cruz Biotechnology [Dallas, TX] and anti-human DSG2 polyclonal antibody AF947 from R&D Systems [Minneapolis, MN]). (E) Transduction of 293 cells with HDAd5/35++GFP and HDAd5/3+GFP in the absence and presence of human (huRBCs) and rhesus (rhRBCs) RBCs (details are provided in the Materials and methods).

Techniques: Expressing, Flow Cytometry, Plasmid Preparation, In Vitro, Transduction, Infection, Virus, Incubation, Recombinant, Staining